Improving engraftment of hepatocyte transplantation using alpha-1 antitrypsin as an immune modulater
No Thumbnail Available
Authors
Lee, Charlotte
Dhawan, Anil
Iansante, Valeria
Filippi, Celine
Mitry, Ragai
Tang, Joanne
Walker, Simon
Fernandez DaCosta, Raquel
Sinha, Siddharth
Hughes, Robin D.
Check for full-text access
Issue Date
2019
Type
Article
Language
Keywords
Alternative Title
Abstract
For patients with non-cirrhotic liver-based metabolic disorders, hepatocyte transplantation can be an effective treatment. However, long-term function of transplanted hepatocytes following infusion has not been achieved due to insufficient numbers of hepatocytes reaching the liver cell plates caused by activation of the instant blood-mediated inflammatory reaction (IBMIR). Our aim was to determine if the natural immune modulator, alpha-1 antitrypsin (AAT), could improve engraftment of transplanted hepatocytes and investigate its mechanism of action. A tubing loop model was used to analyse activation of the IBMIR when human hepatocytes were in contact with ABO-matched blood and 4 mg/ml AAT. Platelet and white cell counts, complement and cytokine expression were analysed. To determine if AAT could improve short-term engraftment, female rats underwent tail vein injection of AAT (120 mg/kg) or water (control) prior to the intrasplenic transplantation of 2 × 10
Description
Citation
Publisher
License
Journal
Journal of Molecular Medicine
Volume
97
Issue
4